Laser photoionization detector for reversed-phase high performance liquid chromatography.

Reversed-phase high-performance liquid chromatography.

Reversed-phase High-performance Liquid Chromatography and Scanning Electron Microscopy

The zein proteins of eight opaque maize mutants were examined by reversed-phase high-performance liquid chromatography (RP-HPLC) and the microstructure of their endosperms by scanning electron microscopy (SEM). The opaque (0) mutants included 01, 05, 09) 010) 011) hI, Oh43JlI and V\ 64AJll. In these opaque mutant maize kernels, the proportion (%) of ex-zein, the major component of the alcohol-e...

Reversed-phase High-performance Liquid Chromatography of Triacylglycerols

The resolution of molecular species of triacylglycerols by reversed-phase high-performance liquid chromatography is discussed. Particular attention is given to the choice of stationary phase, mobile phase, injection solvent, detector and the effect of temperature. The influence of molecular structure on retention time is also explained. Triacylglycerols are major components of the human diet, s...

Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column

The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...

A Reversed Phase High Performance Liquid Chromatographic Method for Determination of Rapamycin

Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...